Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apolipoprotein E Polymorphism and Oxidative Stress in Human Peripheral Blood Cells: Can Physical Activity Reactivate the Proteasome System through Epigenetic Mechanisms?

doi: 10.1155/2021/8869849

Figure Lengend Snippet: Erythrocyte A β , APP, and BACE1 accumulation measured by using a sandwich ELISA kit. The whole blood was collected by volunteers, and erythrocytes were isolated through sequential centrifugations, as described in Materials and Methods. A sandwich ELISA kit was employed to measure the erythrocyte concentration of A β (a), APP (b), and BACE1 (c). The data are shown as the median value with range and are representative of three independent experiments ( n = 3). P values were adjusted with the unpaired t -test: ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001 between the indicated subgroups. GraphPad Prism 7 was used to create the figure.

Article Snippet: Histone deacetylase 6 (HDAC6) was detected in erythrocytes with a competitive ELISA kit (Human Histone Deacetylase 6 (HDAC6) Elisa Kit, Competitive ELISA, MyBioSource, #MBS7254230).

Techniques: Sandwich ELISA, Isolation, Concentration Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apolipoprotein E Polymorphism and Oxidative Stress in Human Peripheral Blood Cells: Can Physical Activity Reactivate the Proteasome System through Epigenetic Mechanisms?

doi: 10.1155/2021/8869849

Figure Lengend Snippet: Erythrocyte Nrf2 and plasma Keap1 quantified by the transcription factor assay and western blot analysis, respectively. (a) Nrf2 was measured in erythrocytes, isolated from the whole blood of healthy volunteers, through a high-throughput assay that combines quick ELISA with a sensitive and specific nonradioactive one for transcription factor activation. By this kind of assay, only the activated Nrf2 is detected (Materials and Methods). (b) Plasma collected from healthy volunteers was considered to identify the presence of the Keap1 through western blot analysis that is shown as the densitometric unit of the bands (Materials and Methods). The data are shown as the mean value ± SD and are representative of three independent experiments ( n = 3). P values were adjusted with the unpaired t -test: ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗∗ P < 0.0001 between the indicated subgroups. GraphPad Prism 7 was used to create the figure.

Article Snippet: Histone deacetylase 6 (HDAC6) was detected in erythrocytes with a competitive ELISA kit (Human Histone Deacetylase 6 (HDAC6) Elisa Kit, Competitive ELISA, MyBioSource, #MBS7254230).

Techniques: Clinical Proteomics, Transcription Factor Assay, Western Blot, Isolation, High Throughput Screening Assay, Enzyme-linked Immunosorbent Assay, Activation Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apolipoprotein E Polymorphism and Oxidative Stress in Human Peripheral Blood Cells: Can Physical Activity Reactivate the Proteasome System through Epigenetic Mechanisms?

doi: 10.1155/2021/8869849

Figure Lengend Snippet: Erythrocyte HDAC6 concentration quantified by using a competitive ELISA kit and circulating expression of miR-195-5p and miR-153-3p. (a) HDAC6 was quantified in erythrocytes, isolated from the whole blood of the total cohort, employing a competitive ELISA kit (Materials and Methods). The data are shown as the mean value ± SD and are representative of three independent experiments ( n = 3). P values were adjusted with the unpaired t -test: ∗∗ P < 0.01 between the indicated subgroups. The expression of miR-195-5p (b) and miR-153-3p (c) was analysed in plasma samples of non-active and active subjects, classified based on ApoE ε 4 polymorphism. The analysis was performed through the miRNeasy Serum/Plasma Mini Kit, as described in Materials and Methods. The relative expression was calculated by the Ct method and normalized on miR-93-5p. Statistical analysis was performed by Kruskal-Wallis (nonparametric) followed by Dunn's multiple comparison test. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 between the indicated subgroups. GraphPad Prism 7 was used to create the figure.

Article Snippet: Histone deacetylase 6 (HDAC6) was detected in erythrocytes with a competitive ELISA kit (Human Histone Deacetylase 6 (HDAC6) Elisa Kit, Competitive ELISA, MyBioSource, #MBS7254230).

Techniques: Concentration Assay, Competitive ELISA, Expressing, Isolation, Clinical Proteomics, Comparison

Journal: Pharmaceutics

Article Title: Novel Multitarget Hydroxamic Acids with a Natural Origin CAP Group against Alzheimer’s Disease: Synthesis, Docking and Biological Evaluation

doi: 10.3390/pharmaceutics13111893

Figure Lengend Snippet: Antioxidant status and HDAC6-inhibiting ability of hydroxamic acids with a natural origin CAP group.

Article Snippet: The HDAC6 enzyme activity was determined by the degree of substrate deacetylation with the fluorescence method based on the study of the substrate deacetylation kinetics in the presence of the enzyme, using a commercially available kit (Enzo Life Sciences—FLUOR DE LYS ® HDAC6 fluorometric drug discovery assay kit, Enzo Biochem, New York, NY, USA).

Techniques: Inhibition, Activity Assay

Journal: Pharmaceutics

Article Title: Novel Multitarget Hydroxamic Acids with a Natural Origin CAP Group against Alzheimer’s Disease: Synthesis, Docking and Biological Evaluation

doi: 10.3390/pharmaceutics13111893

Figure Lengend Snippet: Results of docking of the synthesized compounds 2, 11 – 16, 23 – 25 into the zinc-containing HDAC6 binding site (PDB ID 5EDU); RMSD of reference Trichostatin A is 1.5030 Å.

Article Snippet: The HDAC6 enzyme activity was determined by the degree of substrate deacetylation with the fluorescence method based on the study of the substrate deacetylation kinetics in the presence of the enzyme, using a commercially available kit (Enzo Life Sciences—FLUOR DE LYS ® HDAC6 fluorometric drug discovery assay kit, Enzo Biochem, New York, NY, USA).

Techniques: Synthesized, Binding Assay

Journal: Pharmaceutics

Article Title: Novel Multitarget Hydroxamic Acids with a Natural Origin CAP Group against Alzheimer’s Disease: Synthesis, Docking and Biological Evaluation

doi: 10.3390/pharmaceutics13111893

Figure Lengend Snippet: Docking poses of compounds 11 ( A ), 15 ( B ), and 16 ( C ) in the HDAC6 catalytic domain.

Article Snippet: The HDAC6 enzyme activity was determined by the degree of substrate deacetylation with the fluorescence method based on the study of the substrate deacetylation kinetics in the presence of the enzyme, using a commercially available kit (Enzo Life Sciences—FLUOR DE LYS ® HDAC6 fluorometric drug discovery assay kit, Enzo Biochem, New York, NY, USA).

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: Histone deacetylase inhibitor LMK235 attenuates vascular constriction and aortic remodelling in hypertension

doi: 10.1111/jcmm.14188

Figure Lengend Snippet: IC 50 [μmol/L] values for LMK235 and TSA

Article Snippet: We used fluorogenic HDAC1 (#50061), HDAC2 (#50062), HDAC3 (#50063), HDAC4 (#50064), HDAC5 (#50065), HDAC6 (#50076), HDAC7 (#50067), HDAC8 (#50068) and HDAC9 (#50069) enzyme assay kits from BPS Bioscience (San Diego, CA, USA).

Techniques: